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发明授权
CN101775439B 一种辅助筛选不同千粒重小麦的方法及其专用标记 失效 - 权利终止

一种辅助筛选不同千粒重小麦的方法及其专用标记
摘要：
本发明公开了一种辅助筛选不同千粒重小麦的方法及其专用标记。本发明公布的方法是检测待测小麦基因组DNA中序列2所示核苷酸自5′末端第2708位脱氧核糖核苷酸是A还是G，确定待测小麦的基因型是AA还是GG；所述AA基因型为序列2所示核苷酸自5′末端第2708位脱氧核糖核苷酸为A的纯合体；所述GG基因型为序列2所示核苷酸自5′末端第2708位脱氧核糖核苷酸为G的纯合体；AA基因型为小麦的优异等位变异，其千粒重和/或粒宽高于GG基因型小麦。本标记重复性好、成本低，在提高小麦千粒重(粒宽)的分子标记辅助选择和分子设计育种中具有较好的应用前景。
摘要（英）：

The invention discloses an auxiliary method for screening wheat of different 1000-grain weights and a special marker thereof. The method detects the 2708th deoxyribonucleotide from the end of 5' of the ribotide as showed in Sequence 2 in a genome DNA of the wheat to be detected is A or G to determine that the genotype of the wheat to be detected is AA or GG; the AA genotype is the homozygote of the 2708th deoxyribonucleotide from the end of 5' of the ribotide as shown in Sequence 2 in the genome DNA of the wheat to be detected which is A; the GG genotype is the homozygote of the 2708th deoxyribonucleotide from the end of 5' of the ribotide as shown in Sequence 2 in the genome DNA of the wheat to be detected which is G; the AA genotype is an excellent allelic variation of the wheat, the 1000-grain weight and/or the grain width of the AA genotype wheat is higher than the GG genotype wheat. The marker has good repeatability and low cost and has good application prospect in the auxiliary selection of the molecular marker for improving the 1000-grain weight (particle width) of the wheat and in molecular design breeding.

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