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CN101768637A 用于同时检测线粒体DNAA1555G和C1494T突变的试剂盒及其使用方法 失效 - 权利终止

用于同时检测线粒体DNAA1555G和C1494T突变的试剂盒及其使用方法
摘要：
本发明提供一种用于同时检测与母系遗传药物性耳聋相关的线粒体DNAA1555G和C1494T突变的试剂盒及其使用方法。所述试剂盒包括提取样本基因组DNA的试剂、PCR扩增反应试剂、引物混合液、阳性对照模板、阴性对照模板。所述试剂盒的使用方法主要包括，以血液、带毛囊的毛发、口腔粘膜刮片或唾液等为样本，采用蛋白酶K消化裂解法提取基因组DNA，然后通过多重等位基因特异性PCR同时检出A1555G和C1494T突变。本发明提供的试剂盒用于检测与母系遗传药物性耳聋相关的线粒体DNA？A1555G和C1494T突变，较单独进行A1555G或C1494T突变的检测更快速、经济、简便，且对设备及环境要求低，利于推广和应用。
摘要（英）：

The invention provides a kit for simultaneously detecting mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and a using method thereof. The kit comprises a reagent for extracting sample genomic DNA, a PCR amplification reactive reagent, a primer mixed liquor, a positive control template and a negative control template. The using method of the kit mainly comprises the following steps: using blood, hair with follicle, oral mucosa doctor blade, saliva, and the like, as a sample; adopting a proteinase K digestion pyrolysis method to extract genomic DNA; and then simultaneously detecting mutations in A1555G and C1494T by multiple allele specific PCR. The kit is used for detecting the mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and is more rapid, economical and simpler than the single detection for mutation in A1555G or C1494T, and the kit has low requirements for equipment and environment and is conducive to promotion and application.

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