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CN101768629A 一种利用限制性内切酶防止PCR污染的方法 无效 - 撤回

一种利用限制性内切酶防止PCR污染的方法
摘要：
本发明提供了一种利用限制性内切酶防止PCR污染的方法。具体地，本发明方法包括步骤(a)将PCR反应体系在IIS型限制性内切酶的酶切温度下保温一段时间，从而消化反应体系中可能存在的污染物；(b)灭活IIS型限制性内切酶；和(c)对PCR反应体系进行退火-延伸-变性的循环，从而获得PCR扩增产物。本发明可简便有效地防止前次PCR产物所造成的污染。
摘要（英）：

The invention provides a method for preventing PCR pollution by using restriction endonucleases. The method comprises the following steps: (a) keeping a PCR reaction system at the enzyme digestion temperature of the Type IIS restriction endonucleases for a period of time, thereby digesting pollutants which possibly exist in the reaction system; (b) inactivating the Type IIS restriction endonucleases; and (c) circularly annealing, extending and modifying the PCR reaction system, thereby obtaining the PCR-amplified products. The invention can conveniently and effectively prevent the pollution caused by the products of the previous PCR.

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