The invention relates to a method for evaluating efficacy of an antler, which takes the bifidobacterium in a gastrointestinal tract as representative of beneficial bacterium and takes staphylococcus as representative of harmful bacterium.The method objectively and quantitatively evaluates the influence of different extraction sites of antler alcohol extract on the growth and metabolism of microorganism in the gastrointestinal tract through obtaining a thermogram and other comprehensive information which can fully reflect the interaction process and has overall expression characteristics, screens the active site with the strongest bacterium-promoting action of the antler and inspects the bacteriostasis of the active side simultaneously. The method has simple operation, easy standardizationand automation of the detection.