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发明公开
CN101768211A 新城疫病毒Italien株HN蛋白和F蛋白及其在基因治疗药物中的应用 无效 - 撤回

新城疫病毒Italien株HN蛋白和F蛋白及其在基因治疗药物中的应用
摘要：
本发明涉及一种新城疫病毒Italien株HN蛋白和F蛋白及其在基因治疗药物中的应用，包括NDV？Italien株的HN基因和F基因的cDNA以及NDV？Italien株的HN蛋白和F蛋白氨基酸序列。本发明的有益效果是：得到了可以单独或串联表达NDV？Italien株HN和F基因的真核表达质粒或重组病毒颗粒，使这两种基因能够在真核细胞，包括人体的肿瘤细胞、转化细胞或异常细胞中同时表达其编码产物HN蛋白和F蛋白，并且这两种蛋白可以导致感染细胞与细胞之间融合形成多核巨细胞，最终产生细胞死亡的细胞毒效应。
摘要（英）：

The invention relates to HN protein and F protein of a newcastle disease virus Italien strain and application thereof in a gene therapy medicament. The HN protein and F protein comprise cDNA of HN genes and F genes of the NDV Italien strain and HN protein and F protein amino acid sequences of the NDV Italien strain. The HN protein and F protein have the advantages that eukaryotic expression plasmids or recombinant virus particles capable of expressing the HN and F genes of the NDV Italien strain separately or in series can be obtained so that the two genes can express the HN protein and the F protein of a product coded by the genes at the same time in eukaryotic cells comprising human tumor cells, transformed cells or abnormal cells, and the two proteins can cause fusion between infected cells to form multinucleated giant cells so as to finally produce cell toxin effect of cell death.

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