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发明公开
CN101768207A 一种蛋白质复合物或复合体的三维凝胶电泳分离方法 无效 - 撤回

一种蛋白质复合物或复合体的三维凝胶电泳分离方法
摘要：
本发明公开了一种蛋白质复合物或复合体的三维凝胶电泳分离方法。该方法包括下述步骤：1)将蛋白质混合物进行非变性聚丙烯酰胺凝胶电泳方法分离，然后将其染色。2)切下步骤1)中得到的含目标蛋白质复合物或复合体的凝胶条带，并将其脱色和脱水。3)用蛋白质变性溶液浸泡步骤2)中处理过的凝胶条带，将其充分碾碎后直接进行二维凝胶电泳方法分离。本发明的方法无需传统蛋白质复合物或复合体三维凝胶电泳方法中的蛋白质电洗脱和蛋白质沉淀操作过程，从而避免了蛋白质损失及蛋白质的体外化学修饰；且本发明的方法操作简单，无需另外设备。实验证明，本发明的方法产生了很好蛋白质复合物或复合体的电泳分离效果。
摘要（英）：

The invention discloses a protein complex or compound three-dimensional gel electrophoresis separating method. The method comprises the following steps: 1) separating the protein complex by non-denatured polyacrylamide gel electrophoresis method and then dyeing the protein complex; 2) chopping the gel strips containing the target protein complex or compound obtained in step 1), and decoloring and dehydrating; and 3) soaking the gel strips processed in step 2) by protein denaturation solution, adequately pulverizing and directly performing separation by two-dimensional gel electrophoresis method. The method in the invention dispenses with protein electroelution and protein deposition operating procedures in traditional protein complex or compound three-dimensional gel electrophoresis method, thus avoiding protein loss and protein in vitro chemical decoration; and the method in the invention is simple to operate and needs no additional equipment. Proved by experiments, the method in the invention achieves good protein complex or compound electrophoresis separating effect.

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