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发明公开
CN101768205A 一种聚乙二醇修饰干扰素反应液中回收干扰素的方法 无效 - 撤回

一种聚乙二醇修饰干扰素反应液中回收干扰素的方法
摘要：
本发明属于聚乙二醇修饰干扰素反应液中的各组分分离纯化领域，具体涉及一种聚乙二醇修饰干扰素反应液中回收干扰素的方法。本发明包括如下步骤：a、将聚乙二醇修饰干扰素反应液用酸或碱调至pH值位于5.0～8.0之间；b、将上述所得反应液置换到等pH加样缓冲液，调节加样缓冲液离子强度，再经离子交换层析，使聚乙二醇修饰干扰素穿透，而游离干扰素被吸附；c、将上述离子交换层析柱处理再生后，低盐洗脱，所得洗脱峰即为回收的游离干扰素。本发明中的方法不影响PEG化IFN的收率以及纯度，同时从反应液中所回收的IFN的各项质控指标应与对照品保持一致，能够重复利用。
摘要（英）：

The invention relates to a method for recovering interferon from a polyethylene glycol modified interferon reaction liquid, belonging to the field of separating and purifying each component in the polyethylene glycol modified interferon reaction liquid. The method of the invention comprises the following steps: a, regulating the pH value of the polyethylene glycol modified interferon reaction liquid to 5.0-8.0 with acid or alkali; b, replacing the obtained reaction liquid into a sample loading buffer liquid with the same pH, regulating the ion strength of the sample loading buffer liquid, and carrying out ion-exchange chromatography so that the polyethylene glycol modified interferon penetrates and the dissociated interferon is absorbed; and c, after treating and regenerating the ion-exchange chromatography columns, eluting with low salt to obtain the eluting peak which is the recovered dissociated interferon. The method of the invention does not affect the yield and purity of PEG IFN, and simultaneously, each quality control index of the recovered IFN from the reaction liquid is consistent with a comparison product, and the IFN can be recycled.

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