The invention provides an injection packaging preparation of thymosin pha1 microspheres, comprising thymosin pha1 PLGA buffer microspheres and injection solvent which are separately packaged, wherein, the volume of the injection solvent is 1-500ml, and the dose of the thymosin pha1 buffer microspheres is 1-5000mg; the injection solvent is prepared by the excipients according to the following weight ratio: 0.5-50 parts of thickener, 5-200 parts of protective agent, 0-50 parts of pH regulator, 0-50 parts of iso-osmotic /isotonic regulator, 0-50 parts of solubilizer or cosolvent, 0-20 parts of antioxidant, 0-10 parts of local pain relief agent, and 0-1000 parts of water for injection. The special injection solvent provided by the invention can suspend and disperse the thymosin pha1 microspheres evenly, without reacting with the thymosin pha1 microspheres, and bonding or complexing; moreover, the nature of the microspheres or the thymosin pha1 can maintain stable, thereby ensuring accuracy and effectiveness of the treatment; additionally, the solvent per se is very stable, therefore, the invention provides a novel thymosin pha1 clinical preparation.