The invention relates to a method for determining the starch content in tobacco, which comprises the following steps: weighing a dried and smashed tobacco sample, placing in a beaker, adding distilled water for boiling, cooling, then setting the volume by using the distilled water, shaking up, filtering and preparing a sample reagent; setting the volume of 75-85% phosphoric acid by using the distillated water, adding a 22% polyoxyethylene lauryl ether reagent, and preparing a phosphoric acid reagent; weighing potassium iodate and potassium iodide, setting the volume by using the distilled water, adding the 22% polyoxyethylene lauryl ether reagent, and preparing a potassium iodate/potassium iodide reagent; weighing amylase and amylopectin, commonly dissolving in the distilled water, setting the volume and preparing a standard stock solution reagent; and sequentially carrying out sample injection on all the reagents through the program run of a continuous flow analyzer, and determining the starch content in the sample at a certain wavelength. The method can save the reagents, eliminate the potential safety hazard of experimental staff, protect the instrument and lead the determined starch content in the tobacco to be closer to the true value.