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发明授权
CN101717430B 一种重组人组织型纤溶酶原激活剂改构体的纯化方法 有权 质押

一种重组人组织型纤溶酶原激活剂改构体的纯化方法
摘要：
本发明的目的在于提供一种重组人组织型纤溶酶原激活剂改构体(rhM-tPA)的纯化方法，该方法包括的步骤依次为：上清液过滤处理、第一步层析纯化处理、第二步层析纯化处理、第三步层析纯化处理、第四步超滤浓缩处理。该方法对生物反应器中的重组人组织型纤溶酶原激活剂改构体(rhM-tPA)进行三步层析纯化处理，得到rhM-tPA成品。第一步层析纯化处理采用Blue SepharoseTM6Fast Flow亲和层析柱阶梯状洗脱；第二步层析纯化处理采用Zn2+-POROS20MC螯和柱层析；第三步层析纯化处理采用Biosepra Lysin Hyperd亲和柱层析。所述的三步层析纯化处理均采用亲和层析法，过滤处理得到的上清液仅经过简单的处理可得到所需的高纯度活性的rhM-tPA成品；该方法操作简便，成本低廉，具有较大的经济效益。
摘要（英）：

The present invention provides a purification method of a recombinant human tissue type profibrinolysin activator modified body (rhM-tPA). The method sequentially comprises the following steps of: supernatant fluid filtration processing, first chromatography purification processing, second chromatography purification processing, third chromatography purification processing and fourth ultrafiltration concentration processing. The method obtains a finished rhM-tPA product by carrying out three chromatography purification processings on the recombinant human tissue typ profibrinolysin activator modifiedbody (rhM-tPA) in a bioreactor, wherein the first chromatography purification processing adopts Blue Sepharose TM 6 Fast Flow affinity column ladder shape eluting; the second chromatography purification processing adopts Zn2+-POROS 20MC chelating column chromatography; and the third chromatography purification processing adopts Biosepra Lysin Hyperd affinity column chromatography. The three chromatography purification processings all adopt the affinity chromatography, and a needed finished rhM-tPA product with high purity and activity can be obtained only by simply processing the supernatantfluid obtained by filtration processing. The method has simple and convenient operation, low cost and greater economic benefits.

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