A preparation method for a traditional Chinese medicine composition for improving immunity of livestock and poultry relates to a traditional Chinese medicine composition. The preparation method comprises the following steps: taking acanthopanax root, crushing and sifting; adding ethanol to dynamically soaking and performing reflux extraction; depressurizing the supernatant and recycling ethanol from the supernatant until no alcohol smell is detected; adding ethanol of low concentration to be prepared into crude drug; centrifuging and putting supernatant on macro-porous absorption resin column; using water to elute reaction negativity and successively eluting by ethanol, then collecting ethanol and eluted part of ethanol and combining to obtain acanthopanax total glucoside; taking astragalus root and extracting with ethanol, then mixing with acanthopanax root dregs; filtering and recycling ethanol from filter liquid to obtain total polysaccharide; combining acanthopanax total glucosidewith total polysaccharide and filling into bottles, then sterilizing to obtain injection liquid. The invention uses the technical solution in combination with ethanol extraction method and water extraction method to extract acanthopanax total glucoside, total polysaccharide and astragalus root polysaccharide. The acanthopanax total glucoside, total polysaccharide and astragalus root polysaccharide are added with excipient and processed by common procedures or added with pharmaceutically acceptable excipient to be made into clinically acceptable dosage forms.