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发明授权
CN101696281B 一种具有荧光检测功能的分子印迹微凝胶的制备方法 失效 - 权利终止

一种具有荧光检测功能的分子印迹微凝胶的制备方法
摘要：
本发明公开了一种具有荧光检测功能的分子印迹微凝胶的制备方法。该方法是将马来酸酐酯化淀粉-喹啉偶联物、丙烯酰胺、N，N-亚甲基双丙烯酰胺和茶碱溶于二甲基亚砜中，得到混合溶液；向混合溶液中加入甲醇，室温下搅拌2～4h；在保护气体氛围中，加入偶氮二异丁腈，60～70℃下反应8～12h，分离，得到凝胶化产物；将凝胶化产物洗脱除去模板分子茶碱，得到分子印迹微凝胶。本方法具有成本低、操作简便快捷、灵敏度高等优点，且制得的分子印迹微凝胶具有较好的识别性能及优良的生物降解性和生物相容性，在药物检测领域具有潜在的应用价值。
摘要（英）：

The invention discloses a method for preparing molecularly imprinted microgel with fluorescence detection function, which comprises the following steps: dissolving maleic anhydride esterified starch-quinoline conjugate, acrylamide, N,N-methylenebisacrylamide and theophyllinum in dimethyl sulfoxide to obtain mixed solution; adding methanol to the mixed solution, and stirring for 2 to 4h at room temperature; at atmosphere of a protective gas, adding azodiisobutyronitrile, reacting at a temperature of between 60 and 70 DEG C for 8 to 12h, separating, and obtaining a gelling product; and eluting the gelling product to remove template molecule theophyllinum to obtain the molecularly imprinted microgel. The method has the advantages of low cost, simple, convenient and quick operation, high sensitivity and the like; and the prepared molecularly imprinted microgel has good identification performance and excellent biodegradability and biocompatibility, and has potential application value in the field of medicament detection.

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