The invention discloses a method for the tissue culture of populus deltoids forest 101, which is characterized by comprising the steps of (1) explant disinfection (2) explant selection; (3) cluster bud induction and differentiation of explant; (4) propagation culture; (5) rooting culture; and (6) seedling hardening and transplanting. The method for the isolated culture of the populus deltoids forest 101 has the advantage of high reproduction rate.