The invention discloses an artemisia scoparia extractive and a production method and applications thereof. The artemisia scoparia extractive contains 25-75 percent of a flavonoid component; the production method of the artemisia scoparia extractive comprises the following steps: 1. crudely extracting the overground part or herba of the artemisia scoparia by water or ethanol; 2. adding anhydrous ethyl alcohol to the extracting solution after filtration and concentration, standing and precipitating, and filtering the sediment to obtain ethanol solution; 3. concentrating the obtained ethanol solution, adjusting the pH value, conducting adsorption on a macroporous resin and/or polyamide resin chromatographic column; 4. conducting gradient elution by 10%-90% ethanol, collecting the ethanol eluent and merging; and 5. concentrating the eluent, recovering the ethanol, and drying under reduced pressure, thus obtaining the required artemisia scoparia extractive. Proved by the experimental study, the artemisia scoparia extractive has remarkable effects on resisting influenza viruses, hepatitis B virus and human immunodeficiency virus, and safe oral drug administration, and can be used for preparing broad-spectrum antiviral drugs for treating influenza, hepatitis B and acquired immune deficiency syndrome.