The invention relates to an artificial light source plant culture bed which comprises a culture layer frame used for placing a culture disc, and is characterized in that the culture layer frame is provided with a light source component for providing illumination for the culture disc. The artificial light source plant culture bed can effectively adapt to the growing demand of the whole period of duration of multiple plants especially under the condition that the illumination intensity can not meet the requirement of plant photosynthesis, and can be mainly used for the places such as a scientific research laboratory, a plant tissue culture room, 'a plant factory' and various facilities needing to supplement artificial illumination.