The invention relates to a registered printing method for recombining and transferring in-line rotary printing. At least one blank area not provided with mesh points or meshes is left on a sizing roller coated with transferring glue, and the transferring glue is not coated on an original film part corresponding to the blank area; after the original film and a substrate are recombined and peeled, the layout of the substrate corresponding to the original film part not coated with the transferring glue keeps the original low-luminance characteristic of the substrate, and positioned and registered color scales are printed on the layout keeping the original low-luminance characteristic of the substrate; and then the subsequent registered printing of each color is performed. Compared with the prior art, the registered printing method in the invention is accurate in registered printing and high in yield, and can save materials and reduce the printing cost.