The invention relates to a method for curing and sterilizing culture media, in particular to a method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris. The method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris is characterized in that the steps of curing and sterilizing are performed in combination; the temperature of curing and sterilization is between 100 and 135 DEG C; the time for curing and sterilization is between 40 and 50 minutes; curing and sterilization are synchronously performed in a double-door tunnel-type pressure steam sterilizer with a front door and a back door; the back door of the double-door tunnel-type pressure steam sterilizer is automatically locked when the front door is fed; and the front door of the double-door tunnel-type pressure steam sterilizer is automatically locked when materials are discharged from the back door. The method solves the problem that the prior curing-sterilizing technology is incapable of treating culture media in large capacity and easy to cause bacterial infection resulting in large-scale mould in the process of treating culture matrixes.