The invention belongs to the technical field of animal genetic engineering, and particularly relates to a growth hormone receptor gene taken as a Chinese grassland red bull excellent slaughter trait molecular marker and an application thereof. The length of a nucleotide sequence of an obtained GHR genetic segment is 489bp and the nucleotide sequence is a part of an exon. A base mutation exists at a 289-position basic group to cause the generation of restrictive fragment length polymorphism. The mutant site is taken as the molecular marker and detected by a PCR-RFLP (polymerase chain reaction-restrictive fragment length polymorphism) method using restriction enzyme BstACI, and detection results are associated with the Chinese grassland red bull slaughter traits so as to provide theoretical basis for selection breeding of Chinese grassland red bull. The invention is characterized in that partial segment of GHR gene associated with the Chinese grassland red bull excellent slaughter traits is obtained, and polymorphism analysis is conducted to the segment for providing the molecular marker for marker assisted selection of the Chinese grassland red bull.