The invention relates to a pulse-activating preparation for injection and a preparation method thereof. The pulse-activating preparation contains red ginseng, ophiopogon root and Chinese magnoliavine fruit and is mainly used for treating deficiency of both qi and yin, cardiopalmus caused by feeble pulse, breath shortage, cold limbs, sweating, feeble and impalpable pulse, myocardial infraction, cardiogenic shock and infectious shock. The invention has more stable preparation quality and simple and convenient preparation processes, is safe and reliable, and provides the reasonable and realisticpreparation method for large-scale production of Chinese injection preparation.