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发明授权
CN101580878B 鉴别可溶性酸性转化酶基因等位变异的甜高粱材料的分子标记 失效 - 权利终止

鉴别可溶性酸性转化酶基因等位变异的甜高粱材料的分子标记
摘要：
本发明涉及一种鉴别可溶性酸性转化酶基因等位变异的甜高梁材料的分子标记，属于生物技术领域。该分子标记用如下引物扩增而来：正向引物：5-TTGTCTGCTTGTATATCACC-3，反向引物：5-CTTGTACTCTTGTCGATCGAGC-3，该引物能将鉴别出甜高粱品种中的可溶性酸性转化酶基因的等位变异类型：Sai-1a、Sai-1b Sai-1c，通过发明人的研究发现，各种等位变异类型与品种的蔗糖含糖量显著相关，通过验证证明，Sai-1a类型与低含糖量相关，Sai-1b和Sai-1c与高含糖量有关；本发明获得的分子标记在选择高含糖量潜力的甜高粱品种中的应用将有助于加快育种选择周期。
摘要（英）：

The invention relates to a molecular marker for distinguishing soluble acid invertase allelic variation sweet sorghum material, belonging to the field of biotechnology. The molecular marker is obtained by amplifying the following primers: a forward primer: 5-TTGTCTGCTTGTATATCACC-3 and a reverse primer: 5-CTTGTACTCTTGTCGATCGAGC-3, and the primers can distinguish the types of allelic variation of the soluble acidic invertase gene in the breed of sweet sorghum, namely Sai-1a, Sai-1b and Sai-1c ; by the research of the inventor, all the types of allelic variation are obviously interrelated to the sugar content of sucrose; the verification proves that the type of Sai-1a is interrelated to the low sugar content, and the types of Sai-1b and Sai-1c are interrelated to the high sugar content. The application of the molecular marker obtained by the invention to selecting the breed of sweet sorghum with the potential of high sugar content is beneficial to accelerating the selection period of breeding.

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