The invention provides a manufacturing method of a paraffin tissue chip, which is characterized by comprising the following detailed steps of: numbering the tissues needed, then impregnating paraffin or numbering after marking the tissues needed on the existing paraffin; cutting the tissues into needed size, putting the cut tissues on an ironing table, melting the paraffin thereof at the temperature of 60 to 65 DEG C to obtain tissues to be embedded; taking another paraffin into a paraffin pot, and using an electric furnace to heat and melt the paraffin; putting a square paraffin mold on a glass board, impregnating the tissues to be embedded obtained by the step 1 in the melten paraffin, arranging the tissues at one side of the inner part of the paraffin mold, recording the numbers of tissues of corresponding positions on a positioning board or a positioning paper, reserving a square vacant position at the other side of the inner part of the paraffin mold, pouring the melten paraffin into the paraffin mold along the vacant position till the tissues are impregnated, polymerizing the paraffin, removing the paraffin mold and cutting the obtained paraffin into pieces along the direction parallel to the glass board. The manufacturing method of the paraffin tissue chip has the advantages of cheap price, convenience, fastness, high yield and diverse material-obtaining and can be popularized into each pathology department and relevant research units.