The invention discloses recombinant human keratinocyte growth factor-2 containing eye drops, which are characterized by consisting of recombinant human keratinocyte growth factor-2 as well as an antioxidant, a chelator, a stabilizer, an osmotic pressure adjusting agent, a surfactant and a proper buffer system in a certain proportion. The invention also discloses a method for preparing the eye drops. Pharmacological tests show that eye drops can better promote the repair of damaged corneal epithelium.