A vertical positioning connection structure of a stone bolt is applicable to the vertical positioning connection between underframe cross beam structure of mast type mechanical equipment and a basic concrete beam. The position of the stone bolt along transverse direction and longitudinal direction of a base beam is sacred from restriction of a concrete and the diameter and length of the stone bolt is capable of being adjusted and changed according to specific requirements, thus under the condition of not weakening or as little as possible weakening the section of the concrete base beam and guaranteeing base intensity and integrity, the invention realizes standardized and serialized vertical positioning connection structure with wide applicability between different mast type mechanical equipments structured by different underframe cross beam structure from different factories and prefabricateed concrete bases.