The invention discloses a method for preparing tobacco perfume by adopting organism fermented coffee, and relates to a preparation method of tobacco perfume, which belongs to the technical field of tobacco. The method comprises the following steps: (1) preparing tobacco leaf synthetic medium; (2) selecting the organism producing perfume; (3) performing liquid fermentation or solid fermentation tocoffee by using selected burley tobacco leaf surface dominant bacteria and obtaining the fermented coffee tobacco perfume. The method has simple process and low cost, and the obtained coffee tobacco perfume can be directly used as the perfume of cigarettes. The coffee tobacco perfume has special fragrant characteristic of acid fragrance, thereby lowering the stimulation, richening the smoke gas hiding the mixed gas, leading the smoke gas to be soft and improving the mouthfeel when being added into the cigarettes.