The invention discloses a soybean 11S globulin separation and extraction method. Tris-HCl buffer solution is used as a solvent, the ratio of material to liquid is 1: 21, the extraction temperature is 19 DEG. C, the extraction lasts 1 to 1.5 hours, centrifugation lasts 20min at the speed of 5000r/min, the supernatant fluid is taken with 2mol/L HCl to adjust pH value till 6.4, the temperature of static over night is 4 DEG. C, and the centrifugation last 20min at the speed of 10000r/min after taken out on the next day, and the precipitate is 11S globulin. The extraction yield of 11S globulin is 24 percent.