The invention relates to Chinese toon tissue culture and rapid propagation technology. The technology comprises the following steps: clipping an annual semi-lignified branch with plump axillary buds from a growing plant, clipping the branch into a stem section containing 1 to 2 axillary buds after sterilization, and rapidly inoculating the stem section to a differentiation induction culture medium for differentiation culture; placing the plant obtained through the differentiation culture into a propagation culture medium for propagation and rapid propagation; and when a certain quantity is reached through the propagation, putting the plant into a rootage culture medium for sound seedling rootage culture, and hardening the seedling after the sound seedling takes root. The method carries out the asexual tissue rapid propagation and disinfection through the stem apex and stem section of Chinese toon. The propagated filial generation maintains the good seed nature of the original variety, ensures the seed purity and has no diseases and insects. The technology overcomes the defect of low propagation speed lying in the conventional seed and cut root propagation, enables the artificial control on the culture and growth conditions, is not limited by natural conditions, and has a small amount of drawn material. The technology can realize the industrialized seedling culture and high-efficiency production.