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发明授权
CN101469326B 一种对奇异变形杆菌的ITS特异的核苷酸及其应用 失效 - 权利终止

一种对奇异变形杆菌的ITS特异的核苷酸及其应用
摘要：
本发明涉及一种对奇异变形杆菌(Proteus mirabilis)中16S rRNA-23SrRNA基因的间区(Internal transcribed spacer，简称ITS)特异的的核苷酸，特别是涉及对奇异变形杆菌中的ITS特异的寡核苷酸及其应用，并提供了一种用本发明的寡核苷酸对为引物的PCR检测试剂盒及检测方法，利用该PCR试剂盒对人体及环境中的奇异变形杆菌进行检测，简便快速、特异性好、灵敏度高，可用于食品和临床样品的监督和检测、食物中病原菌检测、细菌学分类及流行病学调查等领域，具有深远的社会效益和较大的经济效益。
摘要（英）：

The invention relates to nucleotide special to an Internal transcribed spacer (ITS for short) of a 16S rRNA-23S rRNA gene in Proteus mirabilis, in particular to oligonucleotide special to the ITS in the Proteus mirabilis and application thereof. The invention also provides a PCR detection reagent taking an oligonucleotide pair as a primer and a detection method thereof. The detection method utilizes the PCR reagent to detect the Proteus mirabilis in human body and the environment, is simple, convenient and quick, has good specificity and high sensitivity, can be used in the fields of supervision and detection of food and clinical samples, detection of pathogen in the food, microbial classification and epidemiological investigation and so on, and has deep social benefit and large economic benefit.

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