The invention relates to a macroporous condensation thiourea chelating resin containing thioureido and a preparation method of the chelating resin, and belongs to the chemical engineering field. The chelating resin comprises the following parts of components by weight: 10-20 parts of the thiourea, 30-50 parts of formaldehyde, 10-20 parts of phenol, 5-10 parts of a pore-forming agent, 5-10 parts of distilled water, 300-500 parts of engine oil and 3-5 parts of baraflo, wherein, the pore-forming agent is NaCl, Na2CO3 and CaCl2, and the phenol is monohydric phenol or polyhydric phenol. The preparation method comprises the steps of weighing, prepolymerization, mixing, polyreaction, curing, heat preservation, dehydration and the like. The method helps effectively prepare the chelating resin into sphere for use while improving the thiourea content of the chelating resin by directly employing the thiourea as a synthetic raw material, and the process for manufacturing the sphere is simple and has low cost, in addition, the method can help prepare the chelating resin into a macroporous type, which expands the application scope.