The present invention relates to a preparation method of rhodamine oil anti-aging summer sleeping mat, including: (1) preparation of rhodamine oil microcapsule: (a) preparation of oil-in-water emulsion, (b) coating, (c) curing; (2) confecting an anti-aging treating agent by 110-130g/L of rhodamine oil microcapsule and 30-40g/L of adhesive; (3) spraying or brushing the anti-aging treating agent onsummer sleeping mat products; (4) drying at 80-95 DEG C for 15min-20min. The summer sleeping mat produced by the method has a good conditioning effect to the female reproductive system, helping to alleviate depression, gradually conditioning the skin and promoting cell regeneration, and having a certain degree of anti-bacterial function.