The invention relates to a gelatin-chitosan spherical porous granular material, a preparation method thereof and a device. The steps are as follows: the first step is to weigh gelatin and chitosan; the second step is to prepare gelatin-chitosan acetic acid solution; the third step is to add the solution into a material storage tank from a feed inlet, control the pressure in the material storage tank to be 0.08-0.5MPa by a pressure gauge and a pressure control valve and lead the solution to evenly flow out at the speed of 10-60 drops/min. Then, the solution is dropped into condensate in a temperature-keeping container at the temperature of minus (10-20) DEG C for condensation, thereby forming the gelatin-chitosan spherical porous granular material with a good sphericity and a uniform particle size. The fourth step is to carry out the separation and the freeze-drying; and the gelatin-chitosan spherical porous granular material is obtained by freeze-drying cross-linking treatment and cleaning. The spherical granular material has uniform particle size distribution, and a large number of mutually communicated micropores are contained therein; furthermore, the spherical granular material has higher specific surface area and can be applied in tissue repair and drug sustained-release.