The invention relates to a guide file credible check based on mobile TPM. The check comprises the following steps: the mobile TPM is initialized; relevant authentication information and check information in a guide process are set; a driving module for the mobile TPM before the start of an operating system at the guide stage is designed and implemented; the driving module is tested according to the operating environment of a DOS operating system; a Part 1 code is arranged at the 0 sector position of a hard disk; an original MBR of the system is moved to a second sector of the hard disk; a Part2 code is arranged at a third sector of the hard disk and a subsequent position thereof; the Part2 code comprises the driving module and a calling interface of the mobile TPM; the credible check is implemented on the reading function of an NEFS file system and files to be read; after the file check is succeeded, the execution is changed from Part2 to an original MBR code. An original process of the system is started for the operation. And the Windows system is ensured to be clean and credible.