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发明公开
CN101413001A 重组人胱抑素C基因及其表达与应用 无效 - 驳回

重组人胱抑素C基因及其表达与应用
摘要：
本发明公开了一种重组人胱抑素C基因及其表达与应用。该截短人胱抑素C基因具有SEQ ID NO：1所示核酸序列；截短人胱抑素C蛋白具有SEQ ID NO：2所示氨基酸序列。本发明运用基因工程技术表达纯化大量稳定的重组可溶胱抑素C蛋白并利用该蛋白获得了一对单抗，进而制备了稳定的胱抑素C诊断试剂及其质控品。解决了我国人胱抑素测定试剂盒自主研发的瓶颈问题。
摘要（英）：

The invention discloses a recombinant human cystatin C gene and expression and application thereof. A truncated human cystatin C gene has a nucleic acid sequence as shown as in SEQ ID NO:1; the truncated human cystatin C protein has an amino acid sequence as shown in SEQ ID NO:2. The invention uses a genetic engineering technique to express and purify a great deal of stable recombinant soluble cystatin C protein and obtains a pair of monoclonal antibodies using the protein to prepare stable cystatin C diagnostic reagents and quality control products thereof, thereby solving bottleneck problems of independent research and development of a cystatin testing reagent box.

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