The invention relates to electrophoresis production equipment with one machine for many colors. The invention designs an electrophoresis tank body as an integrated multi-position tank body, and consists of five parts such as an automatic muff coupling style split pan trolley, an isolating pallet for preventing mixed seepage of residual water, a continuous-flow-typed operating system, and a PLC gas-remote control operating system. Therefore, the technical requirements of coloring different electrophoresis with different colors can be met in the same electrophoresis production line; due to the design of isolating pallet for preventing mixed seepage of residual water in the muff coupling style split pan trolley, the mixed seepage of other colors can be effectively prevented in the process ofspecified coloring on electrophoresis. For the operating system, a fault self-test system and a sound and light alarm system are designed so as to effectively enhance the stability, security and reliability of the equipment in the operating process.