The invention discloses a separating and purifying method of ginkgolides A, B, C and J and bilobalide monomers, which comprises the following processing steps: a. ginkgoic acid is removed from the extractive of ginkgo leafs; b. the total lactones of ginkgo is obtained; c. the preparation solution of the total lactones is prepared; d. the high-performance liquid chromatographic separation of the ginkgolides A, B, C and J and the bilobalide monomers in the total lactones is carried out; and e. the preparation solution of the monomers is processed and the product is dried. By using a preparativehigh-performance liquid chromatographic column to carry out separation and purification and a differential refractive index detector to carry out on-line detection, the invention can synchronously separate and obtain the ginkgolides A, B, C and J and the bilobalide monomers, the purity of which is more than 98 percent, and has the advantages of large preparative scale, high yield, good product quality, low cost and being suitable for industrial production.