The invention provides a whole blood lactate/blood sugar test strip and an integrated blood taking needle packaging the test strip. The test strip is an electrochemical biosensor comprising a testing electrode plate, a cover plate and a reaction reagent layer, a capillary tube cavity body with two open ends is formed between the testing electrode plate and the cover plate, and the reaction reagent layer is formed on the inner wall of the capillary tube cavity body. The integrated blood taking needle for packaging the test strip has a blood taking needle point arranged on one end of a needle bar, and the other end thereof is provided with a U-shaped slot, and the test strip is mounted in the U-shaped slot. The test strip of the invention can automatically soak up trace amount of blood sample and complete the test process of blood lactate/blood sugar in a short time; and the test strip is packaged in the blood taking needle, so the test strip can be taken out for test after collecting the blood sample. The test strip and the blood taking needle of the invention have advantages of providing two detection indexes of blood lactate and blood sugar by one sampling; simple design, low cost, easy processing and making, easy batch production, and convenient carrying and usage.