The invention provides a cell membrane chromatography capable of synchronously sieving active ingredients of antalgic traditional Chinese medicine and determining an opium receptor subtype combined with the active ingredients. The method comprises the following steps of: the establishment of a fingerprint spectrum of extract liquid of angelica dahurica; the primary cultivation and the serial subcultivation of smooth muscle cells in a vas deferens; the combination and the dissociation of effective ingredients; and high-efficient liquid chromatographic determination combining the change of the spectrum. By acting traditional Chinese medicine on different types of smooth muscle cells in the vas deferens, the behavior of dissociation liquid of the traditional Chinese medicine on a high-efficient liquid chromatography column is examined, and the affinity of active ingredients of the traditional Chinese medicine and certain opium receptor subtype can be examined by using opium receptor excitant as a reference to opium receptor antagonist. The method takes an opium receptor on the vas deferens as a sieving model of key links of analgesics effect, can make an active ingredient sieving to the traditional Chinese medicine with abirritation, can also directly determine whether the active ingredients have the affinity with certain opium receptor subtype, and have good application prospect.