The invention discloses a cryopreservation bag used to store liquid nitrogen freezing materials, belonging to the technical field of cryopreservation. The bag comprises a bag body (1) and a bag mouth (2); the bag body (1) is made of nylon or polyester bolting silk which can resist the ultra-low temperature of minus 198 DEG C and the high temperature of 120 DEG C; a keyhole (3) is arranged at either side, 0.5 to 1.0 cm from top margin; the upper end margin of the bag body (1) is turned downward and is sewn into an annular channel (4) with a keyhole (3), forming the bag mouth (2); a drawing rope (5) is arranged in the annular channel (4); thus the bag is formed. The cryopreservation bag used to store liquid nitrogen freezing materials has the advantages that the bag can resist the ultra-low temperature as well as high temperature disinfection; the bag has strong permeability and excellent air permeability, improving the freezing speed of the sample and avoiding the problem of the expansion of the bag; the cryopreservation bag improves the operation efficiency and safety of liquid nitrogen freezing and can be used in scientific research or production field about materials needed to be stored with liquid nitrogen.