The invention provides a method for preparing silica gel with macrospore and microsphere, which can solve the problems in the prior art that separation efficiency is decreased, deep oxidation easily occurs in catalyzing process and yield of target product is reduced. The technical proposal of the invention is as follows: average aperture of the silica gel with macrospore and microsphere is 10 to 60nm; specific surface area is 100 to 400m<2>/g; pore volume is 1.00 to1.60ml/g; and grain diameter is between 50 to 900Mum. The inventions is characterized by cheap price of raw materials, simple working procedure and easy operation as well as greatly reducing of producing cost of the silica gel with macrospore and microsphere, which is in particular suitable for separation and purification of biomacromolecule and Chinese herbal medicine.