The invention relates to a preparation method of a transparent conductive film which is to create a transparent conductive film required for the surface of glass components. The preparation method of the transparent conductive film mainly includes the following steps: the carbon nano-tube pulp is prepared; the prepared carbon nano-tube pulp is formed to a layer of carbon nano-tube pulp on the surface of the glass component; the layer of carbon nano-tube pulp is dried by the heat; the glass component that bears the carbon nano-tube pulp layer is heated to the temperature of 300-500 DEG C underthe protection of nitrogen gas or inactive gas, the temperature is kept for a certain period of time then lowered to room temperature. In this manner, the carbon nano-tube transparent conductive filmis created on the surface of the glass component.