The invention discloses a method which is used for detecting the bacterium Yersini of food-borne coloenteritis with the loop-mediated constant-temperature magnification technology; the method belongs to the bacillus detection technology field and the main technical proposal is that a primer group sequence is designed and the loop-mediated constant-temperature magnification technology is adopted for detection; the loop-mediated constant-temperature magnified product detection methods comprise that 10,000 multiplied g, 5 minutes and room temperature; white sedimentation can be seen at the bottom of the tube in a positive way; visualization reagent is added and the positive result turns green; the process of 1.5 percent agarose gel electrophoresis is implemented and the step-shaped electrophoresis strip is formed; any one of the three loop-mediated constant-temperature magnified product detection methods can be chosen for detection. The method is characterized by accurate detection, strong special and high sensibility and with the method, special object bacillus can be identified fast and accurately and the identification efficiency is increased and the time is saved; in this way, the state that the traditional detection system can not meet the requirements of the ever increasing imports and exports.