The invention relates to a method to cultivate preharvest sprouting resistance hybrid rice sterile line by molecular marker-assisted selection. The steps of the invention are that: step A, hybridization, backcrossing and self crossing are performed by using a preharvest sprouting resistance variety as donor parent and the corresponding maintainer line of sterile line having high combining abilitybut with easy pre-harvest sprouting as recurrent parent; meanwhile the donor parent and the recurrent parent are planted at every season; when choosing among various segregating generations, two microsatellite labeled primers RM447 and RM3754 are used to introduce molecular marker-assisted selection; a plurality of key strains are acquired after two or three generations of backcrossing and five to seven generations of self crossing; step B, the acquired key strains of step A and the sterile line having high combining ability but with easy pre-harvest sprouting are backcrossed for four to seven generations in pairs; during the backcrossing process, the same method of molecular marker-assisted selection as step A is used in selection, then the preharvest sprouting resistance hybrid rice sterile line is cultivated. The invention has the advantages that preharvest sprouting resistance material can be fast and effectively selected; the breeding scale is reduced; the breeding cost is lowered; the preharvest sprouting resistance hybrid rice sterile line is fast selected and bred with high efficiency.