The invention provides a cellsovary granulosa cell anti-aging product, and the preparation method and the application of the product. The invention applies cellsovary granulosa cells in preparation of anti-aging products. The cellsovary granulosa cell anti-aging product of the invention is characterized in that in vitro cultivated and amplified cultivation supernatant of female mammalian animal celsovary granulose cells and/or the cell extract is contained; and one or more than one pharmaceutically acceptable medical excipient and antioxidant are contained. Via the cellsovary granulosa cells,the invention is capable of proliferating and secreting large amounts of a plurality of bioactive substances. The invention adopts granulose cells with extremely high bioavailability and similar to female physiological characteristics to cultivate supernatant and the cell extract of the supernatant. The invention can be taken orally, absorbed by skin or be prepared into vaginal suppository to achieve the purpose of anti-aging.