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发明授权
CN101113468B 云南软米水稻品种毫屁低直链淀粉含量基因位点的分子标记方法 失效 - 权利终止

云南软米水稻品种毫屁低直链淀粉含量基因位点的分子标记方法
摘要：
本发明提供了云南软米水稻品种毫屁低直链淀粉含量基因位点的分子标记方法，属于分子遗传学领域。用标记引物dCAPs-SalI-hp扩增云南软米水稻品种毫屁的DNA，然后用SalI限制性内切酶酶切，如果能得到151bp和145bp两条片断，则标志着云南软米水稻品种毫屁低直链淀粉含量基因位点的存在。通过低直链淀粉含量基因位点的分子标记来检测毫屁及其衍生品种(系)中是否含有该基因位点，可预测其直链淀粉含量水平，大大提高了低直链淀粉含量水稻的选择效率。
摘要（英）：

The invention provides a molecular marking method for low amylose content gene locus of Haopi of Yunnan softpanel rice variety, pertaining to molecular genetics field. The DNA of Haopi of Yunnan softpanel rice variety is increased by a marking primer dCAPs-SalI-hp and then restriction enzyme digestion is done with limited endonuclease SalI. If two fragments respectively with a length of 151bp and145bp can be obtained, the existence of low amylose content gene locus of Haopi of Yunnan softpanel rice variety is proved. The molecular marking for low amylose content gene locus of Haopi of Yunnansoftpanel rice variety is adopted to detect whether Haopi and derivative varieties thereof contain the gene locus and the method can predict the amylose content and greatly improve the efficiency of selection of rice with low amylose content.

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